| PNPT1

Polyribonucleotide nucleotidyltransferase 1 also called Polynucleotide Phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity.That is, it dismantles the RNA chain starting at the 3' end and working toward the 5' end.It also synthesizes long, highly heteropolymeric tails in vivo. It accounts for all of the observed residual polyadenylation in strains of Escherichia coli missing the normal polyadenylation enzyme. The enzyme is encoded by the PNPT1 gene.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.45	1.46	0.89	0.57	0.39	0.28	0.23	0.09
Corrected OD	2.36	1.37	0.8	0.48	0.3	0.19	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human PNPT1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	90-105	102
Cell Lysates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Human PNPT1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	85-96	90-110
	Average (%)	92	96
1 : 4	Range (%)	87-108	105-115
	Average (%)	98	109